Deciphering the Physalis double-layered-lantern 1 mutant provides insights into functional divergence of the GLOBOSA duplicates within the Solanaceae

2014-02-25


作 者:Zhang JS, Li ZC, Zhao J, Zhang SH, Quan H, Zhao M, He CY*
影响因子: 6.736
刊物名称: Plant Physiology
出版年份: 2013
卷: 164  期: 2   页码: 748-764

文章摘要 : 

Physalis develops the ‘Chinese lantern’ trait, also known as inflated calyx syndrome (ICS) that is a morphological novelty. Here, we identified the double-layered-lantern 1 (doll1) mutant in Physalis floridana, a recessive and monofactorial mutation; its corolla and androecium were transformed into the calyx and gynoecium, respectively. Two GLOBOSA-like (GLO) MADS-box paralogous genes PFGLO1 and PFGLO2 were found in P. floridana, while the mutated phenotype was co-segregated with a large deletion harboring PFGLO1 and was complemented by the PFGLO1 genomic locus in transgenic plants, and severe PFGLO1 knockdowns phenocopied doll1. Thus, DOLL1 encodes the PFGLO1 protein and plays a primary role in determining corolla and androecium identity. However, specific PFGLO2 silencing showed no homeotic variation but rather affected pollen maturation. The two genes featured identical floral expression domains but the encoding proteins shared 67% identity in sequences. PFGLO1 was localized in the nucleus when expressed in combination with PFDEF, whereas PFGLO2 was imported to the nucleus on its own. The two proteins were further found to have evolved different interacting partners and regulatory patterns, supporting the hypothesis that PFGLO2 is functionally separated from organ identity. Such a divergent pattern of duplicated GLO genes is unusual within the Solanaceae. Moreover, the phenotypes of the PFGLO1PFGLO2 double silencing mutants suggested that PFGLO2, through genetically interacting with PFGLO1, also exerts a role in the control of organ number and tip development of the second floral whorl. Our results, therefore, shed new light on the functional evolution of the duplicated GLO genes.

原文下载 : 233072.pdf


附件下载:

位于北京西部香山脚下的中国科学院植物研究所是我国系统与进化生物学领域的第一个重点实验室

版权所有 © 系统与进化植物学重点实验室[中国科学院植物研究所]
ICP备16067583号-12 网站管理

联系我们

  • 地址:北京市海淀区 香山南辛村20号
  • 邮编:100093
  • 电话:010-6283 6086
  • 传真:010-6283 6095
  • 电邮:lseb@ibcas.ac.cn

语言切换