Polymorphic chloroplast microsatellite loci in Nelumbo (Nelumbonaceae)
作者:Jian-Hua Xue, Shuo Wang, Shi-Liang Zhou
影响因子:3.159
刊物名称:American Journal of Botany
出版年份:2012
卷:99 期:6 页码:e240-e244
文章摘要:
Premise of the study: To study population genetics, phylogeography, and hybridization of Nelumbo (Nelumbonaceae), chloroplast microsatellite markers were developed.
Methods and Results: Seventeen microsatellite loci were identifi ed from the chloroplast genomes of N. nucifera and N. lutea . Polymorphisms were assessed in three populations of N. nucifera and one population of N. lutea . Nine loci were found to be polymorphic in N. nucifera , and all 17 loci were found to be polymorphic in N. lutea . In N. nucifera , the number of alleles per locus ranged from two to six, and the unbiased haploid diversity per locus ranged from 0.198 to 0.790. In N. lutea , the number of alleles ranged from two to four, and the unbiased haploid diversity per locus ranged from 0.245 to 0.694.
Conclusions: The identifi ed chloroplast simple sequence repeat markers will be useful for the study of genetic diversity, phylogeography, and identifi cation of Nelumbo cultivars.