Development of microsatellite markers for Miscanthus Sinensis (Poaceae) and cross-amplification in other related species

2011-02-05

Development of microsatellite markers for Miscanthus Sinensis (Poaceae) and cross-amplification in other related species

 

作者:Hai-Fei Zhou, Shan-Shan Li, Song Ge*

影响因子:3.452

刊物名称:American Journal of Botany

出版年份:2011

页码:e195-197

 

文章摘要:

Premise of the study : We developed microsatellite loci for the biomass crop Miscanthus sinensis to investigate genetic diversity and population structure of M. sinensis and its closely related species.
Methods and Results : Fourteen microsatellite loci were identifi ed from an enriched genomic library of M. sinensis and tested in one M. sinensis population. The number of alleles per locus ranged from 1 to 15, with a mean of 7.0. The observed and  xpected heterozygosities varied from 0.318 to 0.864 and from 0.424 to 0.901, respectively. Of them, 12 primers could be applied to three other species in Miscanthus ( M. sacchariflorus, M. fl oridulus, and M. lutarioriparius ).
Conclusions : These markers will be important for further analyzing population genetics and evolutionary history, as well as facilitating molecular breeding of Miscanthus sinensis and its related species.

 

原文链接:2011-Zhou Haifei-American Journal of Botany.pdf


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