Instruments

3730xL DNA Analyzer

The 96-capillary 3730xl DNA Analyzer is the Gold Standard for high throughput genetic analysis. In our laboratory, it is mainly applied to DNA fragment analysis, such as traditional DNA sequencing, microsatellites, genetic markers and mutation detection.

Instrument Specifications

1.    In-capillary detection by dual-side illumination. Backside-thinned CCD for maximum optical sensitivity.

2.    Integrated bar code reader, integrated auto sampler and plate stacker.

3.    96 capillaries detect 96 samples in two and a half hours.

4.    Compatible  polymers is POP-7.

5.    Temperature-controlled oven (18-70range) and detection cell heater.

6.    50cm capillary enables read length up to 1100bp and degree of accuracy up to 800bp.

Service Note

User must prepare samples first. Before automatic sampling, the samples are added with 10ul formamide, mildly mixed, and centrifuged. The mixtures are transferred to 96-well plate, and then denaturated more than 2 mins at 95. Please contact the manager to make an appointment.

Charge Standards

288 yuan/plate (96 samples).

Contact: Jin, Wanqing

Tel: 62836494, 62836295

Email: sequencejin@ibcas.ac.cn

MX3000P QPCR Systems

Instrument Specifications

1.    Temperature Uniformity: +/- 0.25°C.

2.    Temperature Ramp Rate: up to2.5°C/second.

3.    Optical System: Four optical channels with user-selected filters that cover a broad range of excitation wavelengths.

4.    Single-scanning fiber optic head design ensures uniformity of well-to-well measurements, eliminating optical variation   and the need for additional signal correction by reference dyes.

5.     Tube/plate formats: 200 μL , 96-well format, optimized for 25 μL sample size.

6.     The trusted chip coupled with it ensures the data safe for electron missing.

7.     Offering unmatched flexibility, the systems are ideal for a wide variety of applications and chemistries.

Software Features

1.    The software, MxPro, provides users with an intuitive interface, quick experiment design, powerful data analysis and easy report generation.

2.    Applied to melting curve analysis with automatic melting temperature (Tm) calculation.

3.    Flexible analysis options: amplification plots, scatter plots, standard curve charts, fluorescence intensity, final call results, melting curve, annealing range and text report.

4.    All results available as raw data, easily export data to Excel, PowerPoint and text files

Service Note

Please contact the manager to you make an appointment and registration. 

Charge Standard

Internal users: 40 yuan/plate; External users: 60 yuan / plate.

Contact: Cai, Qing

Tel: 62836494

Email: caiqing@ibcas.ac.cn

Rotor-Gene 3000 QPCR System

Instrument Specifications

1.     The unique centrifugal rotary: each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling of 500cycle/min. Temperature uniformity is ±0.02oC. Detection is similarly uniform.

2.     Excitation sources: high energy light-emitting diodes; detector: photomultiplier; up to 4 channels with user-selected filters for optical detection.

3.     Powerful software: melting curve analysis, SNP genotyping, etc.

4.     Offering unmatched flexibility, the system is ideal for a wide variety of Kits designed for Real-time PCR cycler.

5.     Low maintenance and maximum convenience due to robust design. Tubes is 0.2 ml or strip tubes 0.1 ml, rotor-disc is 36 or 72.

Service Note

Please contact the manager to you make an appointment.

Charge Standard

Internal users: 40 yuan/run; External users: 60yuan /run.

Contact: Cai, Qing

Tel: 62836494

Email: caiqing@ibcas.ac.cn

ViiA 7 QPCR System

Instrument Specifications

1.     Temperature uniformity: ±0.5°C, within 30 sec of reaching temperature set point.

2.     Precise quantification: detect changes as small as 1.5-fold in target quantities in singleplex reactions.

3.     Maximum multiplexing: six decoupled excitation and emission filter channels for the greatest number of dye combinations and maximum multiplexing capabilities.

4.     Flexible data collection:  multiple ramp method detection formats provide more flexibility for collecting data during a ramp stage.

5.     Compatible with the full range of TaqMan? Assays: optimized to enable clear, clean data for all TaqMan? Assays, including MicroRNA Assays, Protein Assays, Long Non-coding RNA Assays, and Pri-miRNA Assays.

Software Features

1.     Easy touch screen interface: instrument touch screen provides one-touch protocols

2.     for fast and easy assay setup for a broad range of applications.

3.     User-friendly: easily identifiable icons guide to set up runs and analyze experiments. The graphical interface makes it easy to edit the PCR thermal cycling conditions.

4.     Easy management of large data sets: the embedded gene expression (RQ) study application is designed to analyze 100+ runs, while heat maps and scatter plots provide quick checks on data quality.

5.     Real-time control for genotyping cluster analysis: optimize genotyping run times using a real-time PCR progress monitor to determine the ideal cycle for cluster analysis.

Service note

Please book online and contact the manager before use.

Charge Standard

Internal users: 40 yuan/run; External users: 60yuan /run.

Contact: Meng, Fancheng

Tel: 62836150

Email: mengfanchen@ibcas.ac.cn

Hitachi S-4800 Field Emission Scanning Electron Microscope

Instrument Specifications

1.     Resolution of secondary electron image: 1.0 nm15kv.WD=4mm.

2.     Magnification :20x (low magnification mode) – 800,000x (high magnification mode).

3.     Three-axis (X, Y, Z) motorized specimen stage.

Range of application

Observation of fresh plant samples prepared by fixation, dehydration and mount.

Micromorphologies and microstructures of plant tissues, such as pollen, leaf epidermis and testa.

Charge Standard

Internal users: 200 yuan/hour; External users: 300 yuan/hour.

Contact: Xiao, Yinhou

Tel: 62836089

Email: sem@ibcas.ac.cn

Chemilumiescence imaging System

Instruction specifications

The GeneGnome chemilumiescence imaging system is dedicated to chemiluminescence imagingsuch as Biotin or Digoxin labeled Southern Northern BlotWestern BlotElisa.

1.     The darkroom of the GeneGnome is designed with an autodrawer,and the maximum blot size in is11cm x 8cm.

2.     The chemiluminescence imaging software is easily to learn and multifunctional. A very simple set-up process means a single ‘click’ will automatically capture a high quality image .

3.     The GeneGnome is optimised for highly sensitive imaging of chemiluminescence blots and plates (ECLSuperSignalLumiGlo) using the integral cooled high resolution CCD camera.

4.     The GeneGnome is a mini-darkroom, and no film is required.

Contact: Meng, Fanchen

Tel: 62836150

Email: mengfanchen@ibcas.ac.cn

Zeiss Axio Imager 1 Upright light microscope

Instrument specification

Made by Zeiss Company, the Axio Imager 1 microscope has excellent optics and homogeneous illumination in both transmitted light and fluorescence applications. Its apptications contain : Brightfield(H), Darkfield9(D), Differential Interference Contrast(DIC) and Fluorescence observation.

Charge Standard

Internal users: 20 yuan/hour for Brightfield observation, 40 yuan/hour for Fluorescence observation.

External users: 30 yuan/hour for Brighfield observation, 60 yuan/hour for Flourescence oberservation.

Service note

Please book online and contact the manager before use.

Contact: Wen, Jie

Tel: 62836437

Email: jwen@ibcas.ac.cn

Bio-RAD 2-D Electrophorosis System

 

 

Instrument specification

1.     The 1-D electrophorosis system is the PROTEAN IEF CELL.

2.     The 2-D electrophorosis system consists of the Mini-PROTEAN Tetra Cell1-4  8.6×6.8cm precast gels or 8.3 ×7.3cm handcast gels)、the PROTEAN II xi cell1-4  18.3 ×20 cm gels )、PROTEAN Plus Dodeca Cell1-12  20×20.5cm handcast gels or 25×20.5cmprecast gels.

3.     The Molecular Imager PharosFX Plus System includes 488nm/635nm external laser consists of 532nm internal laser, Quantity One software, PDQuest advanced software, 605DF50 and 640DF35 fluorescence and phosphor imaging filters.

Service note

Please book online first and contact the manager before use.

Contact: Meng, Fanchen

Tel: 62836150

Email: mengfanchen@ibcas.ac.cn

The BioLogic DuoFlow Pathfinder 20 System

Instrument specification

The BioLogic DuoFlow Pathfinder 20 system includes:

1.     F10 workstation0.01-20.0 ml/min3500 psi (23 MPa) at 10 ml/min

2.     AVR7-3 sample inject valve: 0.1-5ml

3.     BioLogic Maximizer valve system includes 750 ul BioLogic Maximizer and 263 ul MX-1 Mixerwhich are gradient mixers with barrel extenders to ensure accurate gradient formation at higher flow rate;

4.     BioLogic QuadTec UV/Vis detector for simultaneous monitoring of four wavelengths ranging from 190740 nm (Accuracy: ±1nm\ Detecting range: 0.00001-4.00000AU)

5.     BioLogic DuoFlow pH monitor for real-time monitoring of pH conditions during a run, PH 0-14,0.01 PH unit

6.     BioFrac fraction collector with a variety collection modes provides X-Y motion drop dispensing over a rack and the rack accommodates 90×2 EP tubes

7.     BioLogic DuoFlow Conductivity monitor0-500 ms/cm.

Service note

Please book Online first and contact the manager before use

Contact: Meng, Fanchen

Tel: 62836150

Email: mengfanchen@ibcas.ac.cn

Optima L-100XP Ultracentrifuge

 

Instrument Specifications

1.     Maximum speed 100,000rpm, Maximumg-force 802,400 x g (Type 100 Ti).

2.     Advanced software Optima eXpertTM calculations, simulations, references.

3.     Speed control +/- 10 rpm of set speed.

4.     Sample imbalance tolerance +/- 5mL or 10%, whichever is greater.

5.     Drive cooling Air-cooled.

6.     Refrigeration system Thermoelectric-no CFCs,ODCs.

7.     Set temperature 0 to 40 degrees centigrade in 1 degree increments.

8.     Temperature control +/- 0.5 degrees of set.

9.     Ambient operating range 10 to 40 degrees centigrade.

10.    User-settable programs Virtually no limit to number of user programs.

11.     Acceleration/deceleration rates : 10 accel / 11 decel.

12.     Moisture-purging vacuum system.

13.     Centrifuge-head: NVT100, SW40 Ti, Type100Ti, Type70Ti.

Service note

Please book Online first and contact the manager before use

Charge Standard

40 yuan/hour

Contact: Meng, Fanchen

Tel: 62836150

Email: mengfanchen@ibcas.ac.cn